Integration of microfluidic sample preparation with PCR

Integration of microfluidic pattern preparation with PCR detection to research the results of simultaneous DNA-Inhibitor separation and DNA answer trade   On this paper, we utilized a curved-channel microfluidic system to separate DNA from PCR-inhibitor-containing water and concurrently wash them into clear water for detection utilizing a conveyable PCR thermocycler. Environmental DNA […]

Detection and quantification of EGFR T790M mutation

Detection and quantification of EGFR T790M mutation in liquid biopsies by droplet digital PCR  Background: Liquid biopsy allows the identification of targetable cancer mutations in a minimally invasive manner. In patients with advanced non-small cell lung cancer (NSCLC), droplet digital PCR (ddPCR) is increasingly used to genotype the epidermal growth factor receptor (EGFR) gene […]