Detection and quantification of EGFR T790M mutation in liquid biopsies by droplet digital PCR Background: Liquid biopsy allows the identification of targetable cancer mutations in a minimally invasive manner. In patients with advanced non-small cell lung cancer (NSCLC), droplet digital PCR (ddPCR) is increasingly used to genotype the epidermal growth factor receptor (EGFR) gene […]
A systematic review and meta-analysis of discharged COVID-19 patients retesting positive for RT-PCR Background: With the increased number of patients discharged after having COVID-19, more and more studies have reported cases whose retesting was positive (RP) during the convalescent period, which brings a new public health challenge to the world. Methods: We […]
The Affiliation of Age, Intercourse, and RT-PCR Outcomes with the Lymphocyte and Neutrophil Counts in SARS-CoV-2 An infection: A Cross-sectional Evaluation of 1450 Iranian Sufferers with COVID-19 Containment of pandemic infections primarily relies on immediate identification of carriers, achievable by strict surveillance and truthful diagnostic testing. Though molecular identification of extreme […]
References 1.Sewry CA Dubowitz V. Histochemistry and immunocytochemistry of muscle in health and disease. In: Karpati G Hilton-Jones D Griggs RC, eds. Disorders of Voluntary Muscle, 7th ed. New York: Cambridge University Press, 2001:257 2.Pegoraro E Mancias P Swerdlow SHet al. . Congenital muscular dystrophy with primary laminin alpha2 (merosin) deficiency presenting as inflammatory myopathy. Ann Neurol 1996;40:782–91 3.Marbini A Bellanova MF Ferrari Aet al. . Immunohistochemical study of merosin-negative congenital muscular dystrophy: Laminin alpha 2 deficiency in skin biopsy. Acta […]
This is the third edition of an established two volume text covering all aspects of human muscle disease. Since the second edition in 1994 rapid advances in the molecular genetic understanding of a range of muscle diseases have occurred. Indeed some diseases, such as muscle channelopathies, have only been fully […]
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents. Following solubilization, the material is separated by SDS-PAGE and the antigens are electrophoretically […]
SIITUB The “SIITUB” is intended as a reference to briefly present the experimental methods frequently used in biochemistry. These are not experimental protocols as such. For each of the methods, it presents the basic principles, methods and devices, the main applications to biochemistry, etc. The SIITUB is under “permanent construction”. […]
