Immunohistochemical analysis of canine muscle cells

Immunohistochemistry is an indispensable tool in the assessment and characterization of lineage-specific differentiation of grafted cells in cell-based-therapy. This strategy is under investigation for the treatment of many muscle disorders and different animals such as dogs are used as models to study the tissue regeneration. The aim of the present study was to characterize an antibody panel for the analysis of canine muscle cells, useful in routinely processed formalin-fixed paraffin-embedded tissues.

Overall, 12 antibodies (8 mouse monoclonal and 4 goat polyclonal), validated for use on human tissues tested for cross-reactivity on canine smooth muscle (bladder, intestine, and uterus), skeletal muscle and heart. Specific staining was achieved with eight antibodies, of which six were cytoplasmic markers (desmin, HDAC8, MHC, SMA, Troponin I and Troponin T) and two were cardiac nuclear markers (GATA-4 and Nkx-2.5). This antibody panel may be useful not only for the evaluation of cell-based therapies in muscle disorders, but also for the evaluation of canine soft tissue neoplasms in veterinary pathology.

20T Rapid Canine Brucella Ab Test Kit VivaDiag Pro Antigen test PAL-500-461 Random Hexamers 100 µg Covid-19 Antigen Panbio Covid antigen kits InScience Antigen Rapid Test Covid-19 Antigen Panbio Nasal ELISA kit plasmid 3 plasmid bp plasmid cloning plasmid def plasmid dna isolation plasmid gene expression plasmid hr plasmidin plasmid map plasmido plasmid purification plasmids bioshock plasmids definition plasmid sequence plasmids function plasmid structure plasmid transformation plasmid vector riaa certification riaa eq ria checkpoint riad ria federal credit union rials rialto california rialto homes rialto theater tucson rialto water services pay bill riana riann steele riant rias gremory dxd ria vr80 ria vs bd serum bnp serum ferritin serum institute serum ldh serum lite serum plugin serum presets serum rpr