Muscle disease

This is the third edition of an established two volume text covering all aspects of human muscle disease. Since the second edition in 1994 rapid advances in the molecular genetic understanding of a range of muscle diseases have occurred. Indeed some diseases, such as muscle channelopathies, have only been fully […]

Immunoblotting

Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents. Following solubilization, the material is separated by SDS-PAGE and the antigens are electrophoretically […]

Immuno Dtection

SIITUB The “SIITUB” is intended as a reference to briefly present the experimental methods frequently used in biochemistry. These are not experimental protocols as such. For each of the methods, it presents the basic principles, methods and devices, the main applications to biochemistry, etc. The SIITUB is under “permanent construction”. […]