A systematic review and meta-analysis of discharged COVID-19 patients retesting positive for RT-PCR
Background: With the increased number of patients discharged after having COVID-19, more and more studies have reported cases whose retesting was positive (RP) during the convalescent period, which brings a new public health challenge to the world.
Methods: We searched PubMed, Web of Science, The Cochrane Library, CNKI, WanFang and VIP from December 1, 2019 to December 31, 2020. The included studies were assessed using JBI critical appraisal tools and Newcastle-Ottawa Scale. The RP rate of discharge patients was analyzed by a meta-analysis. We adhered to PRISMA reporting guideline.
Findings: We have included 117 studies with 2669 RP participants after discharge. The methodological quality of 66 case reports were low to high, 42 case series and 3 cohort study were moderate to high, 3 case-control studies were moderate and 3 cross-sectional studies were low to moderate. The clinical manifestations of most RP patients were mild or asymptomatic, and CT imaging and laboratory examinations were usually normal. The existing risk factors suggest that more attention should be paid to sever patients, elderly patients, and patients with co-morbidities. The summary RP rate was 12·2% (95% CI 10·6-13·7) with high heterogeneity (I2 = 85%).
Interpretation: To date, the causes and risk factors of RP result in discharged patients are not fully understood. High-quality etiological and clinical studies are needed to investigate these issues to further help us to make strategies to control and prevent its occurrence.
2×Taq PCR Master Mix(with dye) |
K1034-1 |
ApexBio |
1 ml |
EUR 73 |
2×Taq PCR Master Mix(with dye) |
K1034-100 |
ApexBio |
100×1 ml |
EUR 792 |
2×Taq PCR Master Mix(with dye) |
K1034-20 |
ApexBio |
20×1 ml |
EUR 282 |
2×Taq PCR Master Mix(with dye) |
K1034-5 |
ApexBio |
5×1 ml |
EUR 119 |
2×Taq PCR Master Mix(with dye) |
K1034-50 |
ApexBio |
50×1 ml |
EUR 514 |
2 × Taq Master Mix |
P111-01 |
Vazyme |
5 ml |
EUR 117 |
2 × Taq Master Mix |
P111-02 |
Vazyme |
15 ml |
EUR 146 |
2 × Taq Master Mix |
P111-03 |
Vazyme |
50 ml |
EUR 236 |
2 × Taq Plus Master Mix |
P211-01 |
Vazyme |
5 ml |
EUR 134 |
2 × Taq Plus Master Mix |
P211-02 |
Vazyme |
15 ml |
EUR 193 |
2 × Taq Plus Master Mix |
P211-03 |
Vazyme |
50 ml |
EUR 389 |
2 × Rapid Taq Master Mix |
P222-01 |
Vazyme |
5 ml (5×1ml) |
EUR 120 |
2 × Rapid Taq Master Mix |
P222-02 |
Vazyme |
15 ml (15×1ml) |
EUR 153 |
2 × Rapid Taq Master Mix |
P222-03 |
Vazyme |
50 ml (50 x 1 ml) |
EUR 257 |
2 × Rapid Taq Master Mix |
P222-04 |
Vazyme |
50 ml (10×5ml) |
EUR 257 |
2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn |
W0655-1 |
101Bio |
- |
Ask for price |
2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn |
W0655-5 |
101Bio |
- |
Ask for price |
2× EpiArt HS Taq Master Mix |
EM201-01 |
Vazyme |
1ml |
EUR 145 |
2× EpiArt HS Taq Master Mix |
EM201-02 |
Vazyme |
5 ml (5×1ml) |
EUR 303 |
2× EpiArt HS Taq Master Mix |
EM201-03 |
Vazyme |
15 ml (15×1ml) |
EUR 642 |
2 × Taq Master Mix (Dye Plus) |
P112-01 |
Vazyme |
5 ml |
EUR 117 |
2 × Taq Master Mix (Dye Plus) |
P112-02 |
Vazyme |
15 ml |
EUR 146 |
2 × Taq Master Mix (Dye Plus) |
P112-03 |
Vazyme |
50 ml |
EUR 236 |
Taq PCR Master Mix (2X, Blue Dye) |
BS9295 |
Bio Basic |
1ml |
EUR 84.8 |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9296 |
Bio Basic |
5ml |
EUR 189.2 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9297 |
Bio Basic |
1ml |
EUR 84.8 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9298 |
Bio Basic |
5ml |
EUR 189.2 |
|
2 × Taq Plus Master Mix (Dye Plus) |
P212-01 |
Vazyme |
5 ml |
EUR 133 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-02 |
Vazyme |
15 ml |
EUR 193 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-03 |
Vazyme |
50 ml |
EUR 389 |
Hot Start Taq 2x Master Mix - 500 Reactions |
3296 |
Intact Genomics |
1/EA |
EUR 268 |
2× EpiArt HS Taq Master Mix (Dye Plus) |
EM202-01 |
Vazyme |
1ml |
EUR 145 |
2× EpiArt HS Taq Master Mix (Dye Plus) |
EM202-02 |
Vazyme |
5 ml (5×1ml) |
EUR 303 |
2× EpiArt HS Taq Master Mix (Dye Plus) |
EM202-03 |
Vazyme |
15 ml (15×1ml) |
EUR 642 |
2 × Taq Plus Master Mix II (Dye Plus) |
P213-01 |
Vazyme |
5 ml |
EUR 134 |
2 × Taq Plus Master Mix II (Dye Plus) |
P213-02 |
Vazyme |
15 ml |
EUR 193 |
2 × Taq Plus Master Mix II (Dye Plus) |
P213-03 |
Vazyme |
50 ml |
EUR 389 |
Taq DNA Polymerase with dNTP Mix (6000U) |
9K-001-0018 |
Bio Basic |
6000U |
EUR 1280.62 |
|
Taq DNA Polymerase with dNTP Mix (500U) |
9K-001-0031 |
Bio Basic |
500U |
EUR 292.73 |
|
Taq DNA Polymerase with dNTP Mix (3000U) |
9K-001-0032 |
Bio Basic |
3000U |
EUR 907.39 |
|
Taq DNA Polymerase with dNTP Mix (1000U) |
9K-001-0034 |
Bio Basic |
1000U |
EUR 432.37 |
|
Green Taq Mix |
P131-01 |
Vazyme |
5 ml |
EUR 119 |
Green Taq Mix |
P131-02 |
Vazyme |
15 ml |
EUR 150 |
Green Taq Mix |
P131-03 |
Vazyme |
50 ml |
EUR 248 |
Taqman Master Mix |
M1121-500 |
Biovision |
|
EUR 441 |
Fast Probe Master Mix with Rox (200 rxn) |
31016 |
Biotium |
2x1mL |
EUR 234 |
Description: Minimum order quantity: 1 unit of 2x1mL |
Fast Probe Master Mix with Rox (500 rxn) |
31016-1 |
Biotium |
5x1mL |
EUR 466 |
Description: Minimum order quantity: 1 unit of 5x1mL |
Fast Probe Master Mix with Rox (5000 rxn) |
31016-2 |
Biotium |
50x1mL |
EUR 3871 |
Description: Minimum order quantity: 1 unit of 50x1mL |
amfiSure PCR Master Mix |
P0311-010 |
GenDepot |
2x50 rxns |
EUR 126 |
amfiSure PCR Master Mix |
P0311-025 |
GenDepot |
5X50 rxns |
EUR 165 |
amfiSure PCR Master Mix |
P0311-050 |
GenDepot |
10X50 rxns |
EUR 219 |
amfiSure PCR Master Mix |
P0311-100 |
GenDepot |
10x1ml |
EUR 286 |
amfiSure PCR Master Mix |
P0311-125 |
GenDepot |
15X50 rxns |
EUR 278 |
amfiSure PCR Master Mix |
P0311-200 |
GenDepot |
20x1ml |
EUR 469 |
amfiSure PCR Master Mix |
P0311-250 |
GenDepot |
50x50 rxns |
EUR 752 |
amfiSure PCR Master Mix |
P0311-500 |
GenDepot |
100x50 rxns |
EUR 1258 |
amfiXpand PCR Master Mix |
P0331-010 |
GenDepot |
2X50 rxns |
EUR 151 |
amfiXpand PCR Master Mix |
P0331-025 |
GenDepot |
5X50 rxns |
EUR 273 |
amfiXpand PCR Master Mix |
P0331-050 |
GenDepot |
10X50 rxns |
EUR 448 |
amfiXpand PCR Master Mix |
P0331-250 |
GenDepot |
50x50 rxns |
EUR 1873 |
Taqman Master Mix-iCycler |
M1122-500 |
Biovision |
|
EUR 441 |
Taqman Master Mix-ROX |
M1124-500 |
Biovision |
|
EUR 441 |
Taqman Master Mix-Multiplex |
M1125-500 |
Biovision |
|
EUR 441 |
2 × AceTaq Master Mix |
P411-01 |
Vazyme |
1 ml |
EUR 119 |
2 × AceTaq Master Mix |
P411-02 |
Vazyme |
5 ml |
EUR 158 |
2 × AceTaq Master Mix |
P411-03 |
Vazyme |
15 ml |
EUR 262 |
Taq DNA Polymerase - 5,000 units with separate dNTP Mix |
3245d |
Intact Genomics |
1/EA |
EUR 669 |
Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U) |
9K-001-0003 |
Bio Basic |
500U |
EUR 479.35 |
|
Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U) |
9K-001-0004 |
Bio Basic |
2500U |
EUR 2054.48 |
|
High-Taq DNA Polymerase with 10mM dNTP Mix (250U) |
9K-001-0005 |
Bio Basic |
250U |
EUR 320.14 |
|
High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U) |
9K-001-0006 |
Bio Basic |
4x250U |
EUR 879.98 |
|
High-Taq DNA Polymerase with 10mM dNTP Mix (5000U) |
9K-001-0020 |
Bio Basic |
5000U |
EUR 2399 |
|
Seamless cloning Master Mix (Kit) |
B632219 |
Bio Basic |
40RXN, 40prep |
EUR 266.63 |
|
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 263 |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 291 |
amfiSure Prime PCR Master Mix |
P1311-025 |
GenDepot |
5X50 rxns |
EUR 161 |
amfiSure Prime PCR Master Mix |
P1311-050 |
GenDepot |
10X50 rxns |
EUR 219 |
amfiSure Prime PCR Master Mix |
P1311-125 |
GenDepot |
15X50 rxns |
EUR 273 |
amfiSure Prime PCR Master Mix |
P1311-500 |
GenDepot |
100x50 rxns |
EUR 1238 |
amfiSure Advanced PCR Master Mix |
P2311-025 |
GenDepot |
5X50 rxns |
EUR 165 |
amfiSure Advanced PCR Master Mix |
P2311-050 |
GenDepot |
10X50 rxns |
EUR 223 |
amfiSure Advanced PCR Master Mix |
P2311-125 |
GenDepot |
15X50 rxns |
EUR 282 |
amfiSure Advanced PCR Master Mix |
P2311-250 |
GenDepot |
50x50 rxns |
EUR 752 |
Clinical Utility of Droplet Digital PCR to Monitor BCR-ABL1 Transcripts of Patients With Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia Post-chimeric Antigen Receptor19/22 T-Cell Cocktail Therapy
Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of adult patients with ALL, characterized by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have significantly improved the outcome even though there are still some problems including relapse due to drug-resistant mutations and suboptimal molecular remission depth. Previously, we reported the safety and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy in the treatment of relapsed/refractory (R/R) B-cell neoplasms including cases with Ph+ ALL.
Given possible deeper reaction, more patients were expected to reach optimal minimal residual disease (MRD) response. An alternative method, duplex droplet digital PCR (ddPCR) with high sensitivity was established, which could provide absolute quantification of MRD without the need for calibration curves. Here, we retrospectively collected 95 bone marrow samples from 10 patients with R/R Ph+, who received 19/22 CAR-T-cell cocktail therapy.
Notably, sequential molecular remission for more than 3 months (SMR3), a significant indicator based on ddPCR after CAR-T infusion was established, which was defined as a sequential molecular remission for not <3 months with negative MRD. In this cohort, no recurrence was observed in six patients achieving SMR3, where four of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell regimen.
Unfortunately, the other four patients who did not reach SMR3 relapsed, and did not receive extra specific treatment except CAR-T regimen. To sum up, ddPCR may be an alternative, especially when nucleic acid was insufficient in clinical practice. No achievement of SMR3 may be an early warning of potential relapse after CAR-T and indicating the initiation of other therapies including allo-HSCT.
Quantitative detection and monitoring of Colletotrichum siamense in rubber trees using real-time PCR
Colletotrichum siamense is one of the most important pathogens of rubber trees in Asia. The proper detection and quantification of C. siamense populations in rubber trees are of importance for monitoring the epidemics of the disease. In this study, we developed an ITS-based real-time PCR method to efficiently detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of target DNA and 20 conidia. The real-time PCR protocol recognized all C. siamense isolates collected from three provinces in China, while no amplification was observed with rubber tree and its other pathogens.
Detection and quantification of C. siamense were performed in artificially and naturally infected rubber leaves. We could still detect C. siamense in plant mixes of which only 0.0001% of the tissue infected. An accumulation of C. siamense DNA was observed during the whole infection process at all three leaf phenological stages, suggesting the real-time PCR method can be used to monitor C. siamense development in rubber trees.
Finally, the method allowed the detection of C. siamense in naturally infected and symptomless leaves of rubber trees in the fields. Compared with earlier detection methods, the real-time PCR method is more specific and more sensitive, and will be of great use for studies aiming to gain a better understanding of the epidemiology of Colletotrichum leaf disease, as well as the prediction of disease risk and the control proposal.
Quantitative Measurement of Transposon Copy Number Using the Droplet Digital PCR
Spontaneous proliferation of transposable elements contributes to genetic diversity at varying levels such as somatic mosaicism, genetic divergence in population, and genome evolution. Such genetic diversity is essential for plants’ adaptation to changing environment and serves as a valuable resource for crop improvement. Therefore, measuring the copy number variation of transposable elements with precision and efficiency is important to understand the extent of their proliferation.
Droplet Digital PCR (ddPCR) is an accurate and sensitive technique that allows measurement of copy number variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into thousands of nanoliter-scale droplets. The TaqMan real-time PCR followed by the end-point fluorescence detection enables the quantitative measurement of copy number of template DNAs. Here in this chapter, we describe the step-by-step procedure of ddPCR using EVADE retrotransposon of Arabidopsis as an example.
Comparison of computed tomography (CT) findings with RT-PCR in the diagnosis of COVID-19 in children
Aim: This study aimed to compare chest computed tomography (CT) findings with reverse-transcription polymerase chain reaction (RT-PCR) test results in children with probable or definitive diagnosis of coronavirus disease 2019 (COVID-19).
Methods: In this retrospective archive study, pediatric patients who were followed up in the hospital with a possible or definitive diagnosis of COVID-19 and who had chest CT at presentation were included. CT scan images of the patients were reinterpreted by a pediatric radiologist and compared with their RT-PCR test results.
Results: Of the total of 89 patients, 33 had negative and 56 had positive RT-PCR tests. The presence of pulmonary lesions and consolidation was statistically significantly higher in the RT-PCR negative group than in the RT-PCR positive group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was higher in the RT-PCR positive group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% were significantly higher in the RT-PCR negative group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was found to be statistically significant in the RT-PCR negative group (p = 0.008 and 0.005, respectively).
Conclusion: Chest CT findings may provide some clues in predicting RT-PCR positivity in children with a probable diagnosis of COVID-19. Lobe involvement percentage of up to 25% is a finding in favor of patients with positive RT-PCR test, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation can be interpreted in favor of patients with negative RT-PCR test.
Anti-IL7R antibody (Alexa-fluor 488) |
STJ170020 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
Anti-RPSA Alexa Fluor® 488 |
A4-829-C100 |
ExBio |
0.1 mg |
EUR 310 |
Anti-Hu CD16 Alexa Fluor® 488 |
A4-646-T100 |
ExBio |
100 tests |
EUR 269 |
Goat anti Mouse IgG1 (Alexa Fluor 488) |
43R-1649 |
Fitzgerald |
500 ug |
EUR 570 |
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
Anti-Hu CD72 Alexa Fluor® 488 |
A4-310-T100 |
ExBio |
100 tests |
EUR 269 |
Anti-Bov CD9 Alexa Fluor® 488 |
A4-354-C100 |
ExBio |
0.1 mg |
EUR 269 |
AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
16891 |
AAT Bioquest |
1 mg |
EUR 176 |
|
AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
23153 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
Endoglin/CD105 Alexa Fluor |
FC15024 |
Neuromics |
100 Tests |
EUR 448 |
Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
S0017 |
Affbiotech |
200ul |
EUR 304 |
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
S0018 |
Affbiotech |
200ul |
EUR 304 |
Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
M09406 |
BosterBio |
100ug |
EUR 565 |
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
A4-686-C100 |
ExBio |
0.1 mg |
EUR 269 |
Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
A4-712-C100 |
ExBio |
0.1 mg |
EUR 269 |
Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
A4-730-C100 |
ExBio |
0.1 mg |
EUR 269 |
Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
A4-737-C100 |
ExBio |
0.1 mg |
EUR 269 |
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
Ab8032-001 |
GenDepot |
0.5mg |
EUR 435 |
Anti-LAMP3 antibody (Alexa-fluor 546) |
STJ170005 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
Anti-LAMP3 antibody (Alexa-fluor 647) |
STJ170006 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
Anti-IL3RA antibody (Alexa-fluor 546) |
STJ170010 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
Anti-IL3RA antibody (Alexa-fluor 647) |
STJ170011 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
Anti-CD207 antibody (Alexa-fluor 546) |
STJ170015 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
Anti-CD207 antibody (Alexa-fluor 647) |
STJ170016 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
Anti-IL7R antibody (Alexa-fluor 546) |
STJ170021 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
Anti-IL7R antibody (Alexa-fluor 647) |
STJ170022 |
St John's Laboratory |
100 µg |
EUR 393 |
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
SAM FCM (Alexa Fluor 647) |
abx098902-100tests |
Abbexa |
100 tests |
EUR 1233 |
|
Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
M02316 |
BosterBio |
100ug |
EUR 580 |
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
Alpha Fluor™ 488 Hydroxylamine |
1900 |
AAT Bioquest |
1 mg |
EUR 306 |
|
Streptavidin-Alexa488 (Alexas fluor 488) conjugate |
SV-A488-100 |
Alpha Diagnostics |
100 tests |
EUR 225 |
Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
50336 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
1795 |
AAT Bioquest |
10 mg |
EUR 393 |
|
Mouse IgG1-Alexa 488 conjugate (isotype control) |
20102-101-A488 |
Alpha Diagnostics |
50 Tests |
EUR 263 |
Anti-Hu CD30 Alexa Fluor® 700 |
A7-455-T100 |
ExBio |
100 tests |
EUR 269 |
Anti-Hu CD94 Alexa Fluor® 700 |
A7-727-T100 |
ExBio |
100 tests |
EUR 269 |
Anti-Hu CD56 Alexa Fluor® 700 |
A7-789-T100 |
ExBio |
100 tests |
EUR 269 |
AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
16890 |
AAT Bioquest |
1 mg |
EUR 176 |
|
AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
16892 |
AAT Bioquest |
1 mg |
EUR 176 |
|
AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
23150 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
23158 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
43R-ID005AF |
Fitzgerald |
500 ug |
EUR 338 |
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 364 |
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
43R-ID028AF |
Fitzgerald |
500 ug |
EUR 430 |
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 462 |
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
43R-ID056AF |
Fitzgerald |
500 ug |
EUR 343 |
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
43R-ID060AF |
Fitzgerald |
300 ug |
EUR 425 |
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
43R-IR016AF |
Fitzgerald |
1 mg |
EUR 281 |
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
GPR12 Antibody |
1-CSB-PA008905 |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000 |
GPR12 Antibody |
1-CSB-PA009742LA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
GPR12 Antibody |
44910-100ul |
SAB |
100ul |
EUR 252 |
GPR12 Antibody |
44910-50ul |
SAB |
50ul |
EUR 187 |
GPR12 Antibody |
DF2721 |
Affbiotech |
200ul |
EUR 304 |
Description: GPR12 antibody detects endogenous levels of total GPR12. |
GPR12 antibody |
20R-GR041 |
Fitzgerald |
50 ug |
EUR 656 |
Description: Rabbit polyclonal GPR12 antibody |
GPR12 antibody |
70R-30940 |
Fitzgerald |
100 ug |
EUR 327 |
Description: Rabbit polyclonal GPR12 antibody |
GPR12 antibody |
70R-49804 |
Fitzgerald |
100 ul |
EUR 244 |
Description: Purified Polyclonal GPR12 antibody |
Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
S0005 |
Affbiotech |
200ul |
EUR 376 |
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
S0006 |
Affbiotech |
200ul |
EUR 376 |
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
S0013 |
Affbiotech |
200ul |
EUR 304 |
Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
S0014 |
Affbiotech |
200ul |
EUR 304 |
Mouse pre-microRNA Expression Construct mir-488 |
MMIR-488-PA-1 |
SBI |
Bacterial Streak |
EUR 684 |
|
Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
A6-686-C100 |
ExBio |
0.1 mg |
EUR 269 |
Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
A6-712-C100 |
ExBio |
0.1 mg |
EUR 269 |
Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
A6-730-C100 |
ExBio |
0.1 mg |
EUR 269 |
Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
A6-737-C100 |
ExBio |
0.1 mg |
EUR 269 |
Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
Ab8011-001 |
GenDepot |
1mg |
EUR 334 |
Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
43R-ID012AF |
Fitzgerald |
300 ug |
EUR 410 |
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
GPR12 Polyclonal Antibody |
ES5585-100ul |
ELK Biotech |
100ul |
EUR 279 |
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR12 Polyclonal Antibody |
ES5585-50ul |
ELK Biotech |
50ul |
EUR 207 |
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA |
GPR12 Polyclonal Antibody |
ABP54586-003ml |
Abbkine |
0.03ml |
EUR 158 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ABP54586-01ml |
Abbkine |
0.1ml |
EUR 289 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
GPR12 Polyclonal Antibody |
ABP54586-02ml |
Abbkine |
0.2ml |
EUR 414 |
|
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90 |
Anti-GPR12 antibody |
STJ93328 |
St John's Laboratory |
200 µl |
EUR 197 |
Description: Rabbit polyclonal to GPR12. |
GPR12 Conjugated Antibody |
C44910 |
SAB |
100ul |
EUR 397 |
GPR12 siRNA |
20-abx918427 |
Abbexa |
|
|
|
GPR12 siRNA |
20-abx918428 |
Abbexa |
|
|
|
GPR12 siRNA |
20-abx902268 |
Abbexa |
|
|
|
Anti-GPR12/Gpcr12 Antibody |
A10818 |
BosterBio |
100ul |
EUR 397 |
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human. |
GPR12 Antibody, HRP conjugated |
1-CSB-PA009742LB01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
GPR12 Antibody, FITC conjugated |
1-CSB-PA009742LC01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
GPR12 Antibody, Biotin conjugated |
1-CSB-PA009742LD01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
50337 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
GPR12 Blocking Peptide |
20-abx062679 |
Abbexa |
|
|
|
GPR12 Blocking Peptide |
DF2721-BP |
Affbiotech |
1mg |
EUR 195 |
Polyclonal GPR12 Antibody (Extracellular Domain) |
APR16480G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications: |
Polyclonal GPR12 Antibody (N-Terminus) |
APR16481G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications: |
Rat GPR12 shRNA Plasmid |
20-abx986567 |
Abbexa |
|
|
|
Human GPR12 shRNA Plasmid |
20-abx951900 |
Abbexa |
|
|
|
Mouse GPR12 shRNA Plasmid |
20-abx970621 |
Abbexa |
|
|
|
GPR12 Recombinant Protein (Human) |
RP061035 |
ABM |
100 ug |
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