A systematic review and meta-analysis of discharged COVID-19

A systematic review and meta-analysis of discharged COVID-19 patients retesting positive for RT-PCR

 Background: With the increased number of patients discharged after having COVID-19, more and more studies have reported cases whose retesting was positive (RP) during the convalescent period, which brings a new public health challenge to the world.

 Methods: We searched PubMed, Web of Science, The Cochrane Library, CNKI, WanFang and VIP from December 1, 2019 to December 31, 2020. The included studies were assessed using JBI critical appraisal tools and Newcastle-Ottawa Scale. The RP rate of discharge patients was analyzed by a meta-analysis. We adhered to PRISMA reporting guideline.

 Findings: We have included 117 studies with 2669 RP participants after discharge. The methodological quality of 66 case reports were low to high, 42 case series and 3 cohort study were moderate to high, 3 case-control studies were moderate and 3 cross-sectional studies were low to moderate. The clinical manifestations of most RP patients were mild or asymptomatic, and CT imaging and laboratory examinations were usually normal. The existing risk factors suggest that more attention should be paid to sever patients, elderly patients, and patients with co-morbidities. The summary RP rate was 12·2% (95% CI 10·6-13·7) with high heterogeneity (I² = 85%).

 Interpretation: To date, the causes and risk factors of RP result in discharged patients are not fully understood. High-quality etiological and clinical studies are needed to investigate these issues to further help us to make strategies to control and prevent its occurrence.

 

2×Taq PCR Master Mix(with dye)
K1034-1	ApexBio	1 ml	EUR 73

2×Taq PCR Master Mix(with dye)
K1034-100	ApexBio	100×1 ml	EUR 792

2×Taq PCR Master Mix(with dye)
K1034-20	ApexBio	20×1 ml	EUR 282

2×Taq PCR Master Mix(with dye)
K1034-5	ApexBio	5×1 ml	EUR 119

2×Taq PCR Master Mix(with dye)
K1034-50	ApexBio	50×1 ml	EUR 514

2 × Taq Master Mix
P111-01	Vazyme	5 ml	EUR 117

2 × Taq Master Mix
P111-02	Vazyme	15 ml	EUR 146

2 × Taq Master Mix
P111-03	Vazyme	50 ml	EUR 236

Accuris Taq Master Mix
PR1001-1000	Benchmark Scientific	1 PC	EUR 300.26

Accuris Taq Master Mix
PR1001-200	Benchmark Scientific	1 PC	EUR 121.62

Accuris Taq Master Mix
PR1001-S	Benchmark Scientific	1 PC	EUR 69.79

2 × Taq Plus Master Mix
P211-01	Vazyme	5 ml	EUR 134

2 × Taq Plus Master Mix
P211-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix
P211-03	Vazyme	50 ml	EUR 389

2 × Rapid Taq Master Mix
P222-01	Vazyme	5 ml (5×1ml)	EUR 120

2 × Rapid Taq Master Mix
P222-02	Vazyme	15 ml (15×1ml)	EUR 153

2 × Rapid Taq Master Mix
P222-03	Vazyme	50 ml (50 x 1 ml)	EUR 257

2 × Rapid Taq Master Mix
P222-04	Vazyme	50 ml (10×5ml)	EUR 257

Accuris Taq Plus Master Mix
PR1001-TP-1000	Benchmark Scientific	1 PC	EUR 827.41

Accuris Taq Plus Master Mix
PR1001-TP-200	Benchmark Scientific	1 PC	EUR 242.84

Accuris Taq Plus Master Mix
PR1001-TP-S	Benchmark Scientific	1 PC	EUR 69.79

2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
W0655-1	101Bio	-	Ask for price

2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn
W0655-5	101Bio	-	Ask for price

2× EpiArt HS Taq Master Mix
EM201-01	Vazyme	1ml	EUR 145

2× EpiArt HS Taq Master Mix
EM201-02	Vazyme	5 ml (5×1ml)	EUR 303

2× EpiArt HS Taq Master Mix
EM201-03	Vazyme	15 ml (15×1ml)	EUR 642

2 × Taq Master Mix (Dye Plus)
P112-01	Vazyme	5 ml	EUR 117

2 × Taq Master Mix (Dye Plus)
P112-02	Vazyme	15 ml	EUR 146

2 × Taq Master Mix (Dye Plus)
P112-03	Vazyme	50 ml	EUR 236

Accuris Hot Start Taq Master Mix
PR1001-HS-1000	Benchmark Scientific	1 PC	EUR 490.87

Accuris Hot Start Taq Master Mix
PR1001-HS-200	Benchmark Scientific	1 PC	EUR 169.47

Accuris Taq Master Mix Red Dye
PR1001-R-1000	Benchmark Scientific	1 PC	EUR 300.26

Accuris Taq Master Mix Red Dye
PR1001-R-200	Benchmark Scientific	1 PC	EUR 121.62

Accuris Taq Master Mix Red Dye
PR1001-R-S	Benchmark Scientific	1 PC	EUR 69.79

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 84.8

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 189.2

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 84.8

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 189.2

2 × Taq Plus Master Mix (Dye Plus)
P212-01	Vazyme	5 ml	EUR 133

2 × Taq Plus Master Mix (Dye Plus)
P212-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix (Dye Plus)
P212-03	Vazyme	50 ml	EUR 389

Hot Start Taq 2x Master Mix - 500 Reactions
3296	Intact Genomics	1/EA	EUR 268

2× EpiArt HS Taq Master Mix (Dye Plus)
EM202-01	Vazyme	1ml	EUR 145

2× EpiArt HS Taq Master Mix (Dye Plus)
EM202-02	Vazyme	5 ml (5×1ml)	EUR 303

2× EpiArt HS Taq Master Mix (Dye Plus)
EM202-03	Vazyme	15 ml (15×1ml)	EUR 642

2 × Taq Plus Master Mix II (Dye Plus)
P213-01	Vazyme	5 ml	EUR 134

2 × Taq Plus Master Mix II (Dye Plus)
P213-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix II (Dye Plus)
P213-03	Vazyme	50 ml	EUR 389

Accuris Hot Start Taq Master Mix Red Dye
PR1001-HSR-1000	Benchmark Scientific	1 PC	EUR 490.87

Accuris Hot Start Taq Master Mix Red Dye
PR1001-HSR-200	Benchmark Scientific	1 PC	EUR 169.47

Accuris Hot Start Taq Master Mix Red Dye
PR1001-HSR-S	Benchmark Scientific	1 PC	EUR 69.79

Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018	Bio Basic	6000U	EUR 1280.62

Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031	Bio Basic	500U	EUR 292.73

Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032	Bio Basic	3000U	EUR 907.39

Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034	Bio Basic	1000U	EUR 432.37

Green Taq Mix
P131-01	Vazyme	5 ml	EUR 119

Green Taq Mix
P131-02	Vazyme	15 ml	EUR 150

Green Taq Mix
P131-03	Vazyme	50 ml	EUR 248

Jade? Master Mix
M1105-500	Biovision		EUR 441

Taqman Master Mix
M1121-500	Biovision		EUR 441

Fast Probe Master Mix with Rox (200 rxn)
31016	Biotium	2x1mL	EUR 234
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Probe Master Mix with Rox (500 rxn)
31016-1	Biotium	5x1mL	EUR 466
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Probe Master Mix with Rox (5000 rxn)
31016-2	Biotium	50x1mL	EUR 3871
Description: Minimum order quantity: 1 unit of 50x1mL

amfiSure PCR Master Mix
P0311-010	GenDepot	2x50 rxns	EUR 126

amfiSure PCR Master Mix
P0311-025	GenDepot	5X50 rxns	EUR 165

amfiSure PCR Master Mix
P0311-050	GenDepot	10X50 rxns	EUR 219

amfiSure PCR Master Mix
P0311-100	GenDepot	10x1ml	EUR 286

amfiSure PCR Master Mix
P0311-125	GenDepot	15X50 rxns	EUR 278

amfiSure PCR Master Mix
P0311-200	GenDepot	20x1ml	EUR 469

amfiSure PCR Master Mix
P0311-250	GenDepot	50x50 rxns	EUR 752

amfiSure PCR Master Mix
P0311-500	GenDepot	100x50 rxns	EUR 1258

amfiXpand PCR Master Mix
P0331-010	GenDepot	2X50 rxns	EUR 151

amfiXpand PCR Master Mix
P0331-025	GenDepot	5X50 rxns	EUR 273

amfiXpand PCR Master Mix
P0331-050	GenDepot	10X50 rxns	EUR 448

amfiXpand PCR Master Mix
P0331-250	GenDepot	50x50 rxns	EUR 1873

Taqman Master Mix-iCycler
M1122-500	Biovision		EUR 441

Taqman Master Mix-ROX
M1124-500	Biovision		EUR 441

Taqman Master Mix-Multiplex
M1125-500	Biovision		EUR 441

2 × AceTaq Master Mix
P411-01	Vazyme	1 ml	EUR 119

2 × AceTaq Master Mix
P411-02	Vazyme	5 ml	EUR 158

2 × AceTaq Master Mix
P411-03	Vazyme	15 ml	EUR 262

Taq DNA Polymerase - 5,000 units with separate dNTP Mix
3245d	Intact Genomics	1/EA	EUR 669

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)
9K-001-0003	Bio Basic	500U	EUR 479.35

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)
9K-001-0004	Bio Basic	2500U	EUR 2054.48

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)
9K-001-0005	Bio Basic	250U	EUR 320.14

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)
9K-001-0006	Bio Basic	4x250U	EUR 879.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)
9K-001-0020	Bio Basic	5000U	EUR 2399

Seamless cloning Master Mix (Kit)
B632219	Bio Basic	40RXN, 40prep	EUR 266.63

SYBR Green qPCR Master Mix
HY-K0501	MedChemExpress	5 mL (500 rxns)	EUR 263

RT Master Mix for qPCR
HY-K0510	MedChemExpress	1 mL (100 rxns)	EUR 291

amfiSure Prime PCR Master Mix
P1311-025	GenDepot	5X50 rxns	EUR 161

amfiSure Prime PCR Master Mix
P1311-050	GenDepot	10X50 rxns	EUR 219

amfiSure Prime PCR Master Mix
P1311-125	GenDepot	15X50 rxns	EUR 273

amfiSure Prime PCR Master Mix
P1311-500	GenDepot	100x50 rxns	EUR 1238

amfiSure Advanced PCR Master Mix
P2311-025	GenDepot	5X50 rxns	EUR 165

amfiSure Advanced PCR Master Mix
P2311-050	GenDepot	10X50 rxns	EUR 223

amfiSure Advanced PCR Master Mix
P2311-125	GenDepot	15X50 rxns	EUR 282

amfiSure Advanced PCR Master Mix
P2311-250	GenDepot	50x50 rxns	EUR 752

Clinical Utility of Droplet Digital PCR to Monitor BCR-ABL1 Transcripts of Patients With Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia Post-chimeric Antigen Receptor19/22 T-Cell Cocktail Therapy

 Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph⁺ ALL) accounts for 20-30% of adult patients with ALL, characterized by translocation of t _{(9, 22)}. Tyrosine kinase inhibitors (TKIs) have significantly improved the outcome even though there are still some problems including relapse due to drug-resistant mutations and suboptimal molecular remission depth. Previously, we reported the safety and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy in the treatment of relapsed/refractory (R/R) B-cell neoplasms including cases with Ph⁺ ALL.

Given possible deeper reaction, more patients were expected to reach optimal minimal residual disease (MRD) response. An alternative method, duplex droplet digital PCR (ddPCR) with high sensitivity was established, which could provide absolute quantification of MRD without the need for calibration curves. Here, we retrospectively collected 95 bone marrow samples from 10 patients with R/R Ph⁺, who received 19/22 CAR-T-cell cocktail therapy.

Notably, sequential molecular remission for more than 3 months (SMR3), a significant indicator based on ddPCR after CAR-T infusion was established, which was defined as a sequential molecular remission for not <3 months with negative MRD. In this cohort, no recurrence was observed in six patients achieving SMR3, where four of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell regimen.

Unfortunately, the other four patients who did not reach SMR3 relapsed, and did not receive extra specific treatment except CAR-T regimen. To sum up, ddPCR may be an alternative, especially when nucleic acid was insufficient in clinical practice. No achievement of SMR3 may be an early warning of potential relapse after CAR-T and indicating the initiation of other therapies including allo-HSCT.

 

Quantitative detection and monitoring of Colletotrichum siamense in rubber trees using real-time PCR

 Colletotrichum siamense is one of the most important pathogens of rubber trees in Asia. The proper detection and quantification of C. siamense populations in rubber trees are of importance for monitoring the epidemics of the disease. In this study, we developed an ITS-based real-time PCR method to efficiently detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of target DNA and 20 conidia. The real-time PCR protocol recognized all C. siamense isolates collected from three provinces in China, while no amplification was observed with rubber tree and its other pathogens.

Detection and quantification of C. siamense were performed in artificially and naturally infected rubber leaves. We could still detect C. siamense in plant mixes of which only 0.0001% of the tissue infected. An accumulation of C. siamense DNA was observed during the whole infection process at all three leaf phenological stages, suggesting the real-time PCR method can be used to monitor C. siamense development in rubber trees.

Finally, the method allowed the detection of C. siamense in naturally infected and symptomless leaves of rubber trees in the fields. Compared with earlier detection methods, the real-time PCR method is more specific and more sensitive, and will be of great use for studies aiming to gain a better understanding of the epidemiology of Colletotrichum leaf disease, as well as the prediction of disease risk and the control proposal.

Quantitative Measurement of Transposon Copy Number Using the Droplet Digital PCR

 Spontaneous proliferation of transposable elements contributes to genetic diversity at varying levels such as somatic mosaicism, genetic divergence in population, and genome evolution. Such genetic diversity is essential for plants’ adaptation to changing environment and serves as a valuable resource for crop improvement. Therefore, measuring the copy number variation of transposable elements with precision and efficiency is important to understand the extent of their proliferation.

Droplet Digital PCR (ddPCR) is an accurate and sensitive technique that allows measurement of copy number variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into thousands of nanoliter-scale droplets. The TaqMan real-time PCR followed by the end-point fluorescence detection enables the quantitative measurement of copy number of template DNAs. Here in this chapter, we describe the step-by-step procedure of ddPCR using EVADE retrotransposon of Arabidopsis as an example.

Comparison of computed tomography (CT) findings with RT-PCR in the diagnosis of COVID-19 in children

 Aim: This study aimed to compare chest computed tomography (CT) findings with reverse-transcription polymerase chain reaction (RT-PCR) test results in children with probable or definitive diagnosis of coronavirus disease 2019 (COVID-19).

 Methods: In this retrospective archive study, pediatric patients who were followed up in the hospital with a possible or definitive diagnosis of COVID-19 and who had chest CT at presentation were included. CT scan images of the patients were reinterpreted by a pediatric radiologist and compared with their RT-PCR test results.

 Results: Of the total of 89 patients, 33 had negative and 56 had positive RT-PCR tests. The presence of pulmonary lesions and consolidation was statistically significantly higher in the RT-PCR negative group than in the RT-PCR positive group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was higher in the RT-PCR positive group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% were significantly higher in the RT-PCR negative group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was found to be statistically significant in the RT-PCR negative group (p = 0.008 and 0.005, respectively).

 Conclusion: Chest CT findings may provide some clues in predicting RT-PCR positivity in children with a probable diagnosis of COVID-19. Lobe involvement percentage of up to 25% is a finding in favor of patients with positive RT-PCR test, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation can be interpreted in favor of patients with negative RT-PCR test.

Anti-IL7R antibody (Alexa-fluor 488)
STJ170020	St John's Laboratory	100 µg	EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-RPSA Alexa Fluor® 488
A4-829-C100	ExBio	0.1 mg	EUR 310

Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100	ExBio	100 tests	EUR 269

Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649	Fitzgerald	500 ug	EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD72 Alexa Fluor® 488
A4-310-T100	ExBio	100 tests	EUR 269

Anti-Bov CD9 Alexa Fluor® 488
A4-354-C100	ExBio	0.1 mg	EUR 269

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]
16891	AAT Bioquest	1 mg	EUR 176

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]
23153	AAT Bioquest	300 Tests	EUR 306

Endoglin/CD105 Alexa Fluor
FC15024	Neuromics	100 Tests	EUR 448

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated
S0017	Affbiotech	200ul	EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated
S0018	Affbiotech	200ul	EUR 304

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)
M09406	BosterBio	100ug	EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488
A4-686-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488
A4-712-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488
A4-730-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488
A4-737-C100	ExBio	0.1 mg	EUR 269

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated
Ab8032-001	GenDepot	0.5mg	EUR 435

Anti-LAMP3 antibody (Alexa-fluor 546)
STJ170005	St John's Laboratory	100 µg	EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)
STJ170006	St John's Laboratory	100 µg	EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 546)
STJ170010	St John's Laboratory	100 µg	EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)
STJ170011	St John's Laboratory	100 µg	EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 546)
STJ170015	St John's Laboratory	100 µg	EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)
STJ170016	St John's Laboratory	100 µg	EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 546)
STJ170021	St John's Laboratory	100 µg	EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)
STJ170022	St John's Laboratory	100 µg	EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

SAM FCM (Alexa Fluor 647)
abx098902-100tests	Abbexa	100 tests	EUR 1233

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)
M02316	BosterBio	100ug	EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Alpha Fluor™ 488 amine
1705	AAT Bioquest	1 mg	EUR 306

Alpha Fluor™ 488 Hydroxylamine
1900	AAT Bioquest	1 mg	EUR 306

Alpha Fluor™ 488 NHS Ester
1812	AAT Bioquest	1 mg	EUR 219

Streptavidin-Alexa488 (Alexas fluor 488) conjugate
SV-A488-100	Alpha Diagnostics	100 tests	EUR 225

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)
50336	Alpha Diagnostics	0.5 ml	EUR 225

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]
1795	AAT Bioquest	10 mg	EUR 393

Mouse IgG1-Alexa 488 conjugate (isotype control)
20102-101-A488	Alpha Diagnostics	50 Tests	EUR 263

Anti-Hu CD30 Alexa Fluor® 700
A7-455-T100	ExBio	100 tests	EUR 269

Anti-Hu CD94 Alexa Fluor® 700
A7-727-T100	ExBio	100 tests	EUR 269

Anti-Hu CD56 Alexa Fluor® 700
A7-789-T100	ExBio	100 tests	EUR 269

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]
16890	AAT Bioquest	1 mg	EUR 176

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]
16892	AAT Bioquest	1 mg	EUR 176

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]
23150	AAT Bioquest	300 Tests	EUR 306

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]
23158	AAT Bioquest	300 Tests	EUR 306

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)
43R-ID005AF	Fitzgerald	500 ug	EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID022AF	Fitzgerald	500 ug	EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)
43R-ID028AF	Fitzgerald	500 ug	EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF	Fitzgerald	500 ug	EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-ID056AF	Fitzgerald	500 ug	EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)
43R-ID060AF	Fitzgerald	300 ug	EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-IR016AF	Fitzgerald	1 mg	EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

GPR12 Antibody
1-CSB-PA008905	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody
1-CSB-PA009742LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12 Antibody
44910-100ul	SAB	100ul	EUR 252

GPR12 Antibody
44910-50ul	SAB	50ul	EUR 187

GPR12 Antibody
DF2721	Affbiotech	200ul	EUR 304
Description: GPR12 antibody detects endogenous levels of total GPR12.

GPR12 Antibody
ABD2721	Lifescience Market	100 ug	EUR 438

GPR12 antibody
20R-GR041	Fitzgerald	50 ug	EUR 656
Description: Rabbit polyclonal GPR12 antibody

GPR12 antibody
70R-30940	Fitzgerald	100 ug	EUR 327
Description: Rabbit polyclonal GPR12 antibody

GPR12 antibody
70R-49804	Fitzgerald	100 ul	EUR 244
Description: Purified Polyclonal GPR12 antibody

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated
S0005	Affbiotech	200ul	EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated
S0006	Affbiotech	200ul	EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated
S0013	Affbiotech	200ul	EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated
S0014	Affbiotech	200ul	EUR 304

GPR12
RA30050	Neuromics	50 ug	EUR 618

Mouse pre-microRNA Expression Construct mir-488
MMIR-488-PA-1	SBI	Bacterial Streak	EUR 684

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647
A6-686-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647
A6-712-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647
A6-730-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647
A6-737-C100	ExBio	0.1 mg	EUR 269

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated
Ab8011-001	GenDepot	1mg	EUR 334

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)
43R-ID012AF	Fitzgerald	300 ug	EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

GPR12 Polyclonal Antibody
ES5585-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody
ES5585-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody
ABP54586-003ml	Abbkine	0.03ml	EUR 158
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody
ABP54586-01ml	Abbkine	0.1ml	EUR 289
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody
ABP54586-02ml	Abbkine	0.2ml	EUR 414
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

Anti-GPR12 antibody
STJ93328	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to GPR12.

GPR12 Conjugated Antibody
C44910	SAB	100ul	EUR 397

Gpr12/ Rat Gpr12 ELISA Kit
ELI-09764r	Lifescience Market	96 Tests	EUR 886

GPR12 siRNA
20-abx918427	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

GPR12 siRNA
20-abx918428	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

GPR12 siRNA
20-abx902268	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

Anti-GPR12/Gpcr12 Antibody
A10818	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human.

GPR12 Antibody, HRP conjugated
1-CSB-PA009742LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated
1-CSB-PA009742LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated
1-CSB-PA009742LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)
50337	Alpha Diagnostics	0.5 ml	EUR 225

GPR12 Blocking Peptide
20-abx062679	Abbexa	EUR 272.00 EUR 411.00	1 mg 5 mg

GPR12 Blocking Peptide
DF2721-BP	Affbiotech	1mg	EUR 195

Polyclonal GPR12 Antibody (Extracellular Domain)
APR16480G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)
APR16481G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

Rat GPR12 shRNA Plasmid
20-abx986567	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Human GPR12 shRNA Plasmid
20-abx951900	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Mouse GPR12 shRNA Plasmid
20-abx970621	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

GPR12 ELISA KIT|Human
EF005024	Lifescience Market	96 Tests	EUR 689

GPR12 Recombinant Protein (Human)
RP061035	ABM	100 ug	Ask for price