A systematic review and meta-analysis of discharged COVID-19

Posted on by Lydia

A systematic review and meta-analysis of discharged COVID-19 patients retesting positive for RT-PCR

 Background: With the increased number of patients discharged after having COVID-19, more and more studies have reported cases whose retesting was positive (RP) during the convalescent period, which brings a new public health challenge to the world.

 Methods: We searched PubMed, Web of Science, The Cochrane Library, CNKI, WanFang and VIP from December 1, 2019 to December 31, 2020. The included studies were assessed using JBI critical appraisal tools and Newcastle-Ottawa Scale. The RP rate of discharge patients was analyzed by a meta-analysis. We adhered to PRISMA reporting guideline.

 Findings: We have included 117 studies with 2669 RP participants after discharge. The methodological quality of 66 case reports were low to high, 42 case series and 3 cohort study were moderate to high, 3 case-control studies were moderate and 3 cross-sectional studies were low to moderate. The clinical manifestations of most RP patients were mild or asymptomatic, and CT imaging and laboratory examinations were usually normal. The existing risk factors suggest that more attention should be paid to sever patients, elderly patients, and patients with co-morbidities. The summary RP rate was 12·2% (95% CI 10·6-13·7) with high heterogeneity (I2 = 85%).

 Interpretation: To date, the causes and risk factors of RP result in discharged patients are not fully understood. High-quality etiological and clinical studies are needed to investigate these issues to further help us to make strategies to control and prevent its occurrence.

 

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Clinical Utility of Droplet Digital PCR to Monitor BCR-ABL1 Transcripts of Patients With Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia Post-chimeric Antigen Receptor19/22 T-Cell Cocktail Therapy

 Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of adult patients with ALL, characterized by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have significantly improved the outcome even though there are still some problems including relapse due to drug-resistant mutations and suboptimal molecular remission depth. Previously, we reported the safety and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy in the treatment of relapsed/refractory (R/R) B-cell neoplasms including cases with Ph+ ALL.

Given possible deeper reaction, more patients were expected to reach optimal minimal residual disease (MRD) response. An alternative method, duplex droplet digital PCR (ddPCR) with high sensitivity was established, which could provide absolute quantification of MRD without the need for calibration curves. Here, we retrospectively collected 95 bone marrow samples from 10 patients with R/R Ph+, who received 19/22 CAR-T-cell cocktail therapy.

Notably, sequential molecular remission for more than 3 months (SMR3), a significant indicator based on ddPCR after CAR-T infusion was established, which was defined as a sequential molecular remission for not <3 months with negative MRD. In this cohort, no recurrence was observed in six patients achieving SMR3, where four of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell regimen.

Unfortunately, the other four patients who did not reach SMR3 relapsed, and did not receive extra specific treatment except CAR-T regimen. To sum up, ddPCR may be an alternative, especially when nucleic acid was insufficient in clinical practice. No achievement of SMR3 may be an early warning of potential relapse after CAR-T and indicating the initiation of other therapies including allo-HSCT.

 

Quantitative detection and monitoring of Colletotrichum siamense in rubber trees using real-time PCR

 Colletotrichum siamense is one of the most important pathogens of rubber trees in Asia. The proper detection and quantification of C. siamense populations in rubber trees are of importance for monitoring the epidemics of the disease. In this study, we developed an ITS-based real-time PCR method to efficiently detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of target DNA and 20 conidia. The real-time PCR protocol recognized all C. siamense isolates collected from three provinces in China, while no amplification was observed with rubber tree and its other pathogens.

Detection and quantification of C. siamense were performed in artificially and naturally infected rubber leaves. We could still detect C. siamense in plant mixes of which only 0.0001% of the tissue infected. An accumulation of C. siamense DNA was observed during the whole infection process at all three leaf phenological stages, suggesting the real-time PCR method can be used to monitor C. siamense development in rubber trees.

Finally, the method allowed the detection of C. siamense in naturally infected and symptomless leaves of rubber trees in the fields. Compared with earlier detection methods, the real-time PCR method is more specific and more sensitive, and will be of great use for studies aiming to gain a better understanding of the epidemiology of Colletotrichum leaf disease, as well as the prediction of disease risk and the control proposal.

Quantitative Measurement of Transposon Copy Number Using the Droplet Digital PCR

 Spontaneous proliferation of transposable elements contributes to genetic diversity at varying levels such as somatic mosaicism, genetic divergence in population, and genome evolution. Such genetic diversity is essential for plants’ adaptation to changing environment and serves as a valuable resource for crop improvement. Therefore, measuring the copy number variation of transposable elements with precision and efficiency is important to understand the extent of their proliferation.

Droplet Digital PCR (ddPCR) is an accurate and sensitive technique that allows measurement of copy number variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into thousands of nanoliter-scale droplets. The TaqMan real-time PCR followed by the end-point fluorescence detection enables the quantitative measurement of copy number of template DNAs. Here in this chapter, we describe the step-by-step procedure of ddPCR using EVADE retrotransposon of Arabidopsis as an example.

Comparison of computed tomography (CT) findings with RT-PCR in the diagnosis of COVID-19 in children

 Aim: This study aimed to compare chest computed tomography (CT) findings with reverse-transcription polymerase chain reaction (RT-PCR) test results in children with probable or definitive diagnosis of coronavirus disease 2019 (COVID-19).

 Methods: In this retrospective archive study, pediatric patients who were followed up in the hospital with a possible or definitive diagnosis of COVID-19 and who had chest CT at presentation were included. CT scan images of the patients were reinterpreted by a pediatric radiologist and compared with their RT-PCR test results.

 Results: Of the total of 89 patients, 33 had negative and 56 had positive RT-PCR tests. The presence of pulmonary lesions and consolidation was statistically significantly higher in the RT-PCR negative group than in the RT-PCR positive group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was higher in the RT-PCR positive group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% were significantly higher in the RT-PCR negative group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was found to be statistically significant in the RT-PCR negative group (p = 0.008 and 0.005, respectively).

 Conclusion: Chest CT findings may provide some clues in predicting RT-PCR positivity in children with a probable diagnosis of COVID-19. Lobe involvement percentage of up to 25% is a finding in favor of patients with positive RT-PCR test, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation can be interpreted in favor of patients with negative RT-PCR test.

Anti-LAMP3 antibody (Alexa-fluor 488)
STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)
STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)
STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)
STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-RPSA Alexa Fluor® 488
A4-829-C100 0.1 mg
EUR 310

Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100 100 tests
EUR 269

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]
23153 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]
16891 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Anti-Hu CD72 Alexa Fluor® 488
A4-310-T100 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488
A4-354-C100 0.1 mg
EUR 269

Endoglin/CD105 Alexa Fluor
FC15024 100 Tests
EUR 448

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated
S0017 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated
S0018 200ul
EUR 304

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)
M09406 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated
Ab8032-001 0.5mg
EUR 435

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488
A4-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488
A4-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488
A4-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488
A4-737-C100 0.1 mg
EUR 269

Anti-LAMP3 antibody (Alexa-fluor 546)
STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)
STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 546)
STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)
STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 546)
STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)
STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 546)
STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)
STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

SAM FCM (Alexa Fluor 647)
abx098902-100tests 100 tests
EUR 1233
  • Shipped within 5-10 working days.

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)
M02316 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Alpha Fluor™ 488 amine
1705 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 488 Hydroxylamine
1900 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 488 NHS Ester
1812 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Streptavidin-Alexa488 (Alexas fluor 488) conjugate
SV-A488-100 100 tests
EUR 225

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)
50336 0.5 ml
EUR 225

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]
1795 10 mg
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Mouse IgG1-Alexa 488 conjugate (isotype control)
20102-101-A488 50 Tests
EUR 263

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]
23150 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]
23158 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]
16890 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]
16892 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)
43R-ID005AF 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID022AF 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)
43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-ID056AF 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)
43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-IR016AF 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Anti-Hu CD30 Alexa Fluor® 700
A7-455-T100 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700
A7-727-T100 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700
A7-789-T100 100 tests
EUR 269

GPR12 antibody
20R-GR041 50 ug
EUR 656
Description: Rabbit polyclonal GPR12 antibody

GPR12 antibody
70R-30940 100 ug
EUR 327
Description: Rabbit polyclonal GPR12 antibody

GPR12 Antibody
44910-100ul 100ul
EUR 252

GPR12 Antibody
44910-50ul 50ul
EUR 187

GPR12 Antibody
DF2721 200ul
EUR 304
Description: GPR12 antibody detects endogenous levels of total GPR12.

GPR12 antibody
70R-49804 100 ul
EUR 244
Description: Purified Polyclonal GPR12 antibody

GPR12 Antibody
1-CSB-PA008905
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody
1-CSB-PA009742LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12 Antibody
ABD2721 100 ug
EUR 438

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated
S0005 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated
S0006 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated
S0013 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated
S0014 200ul
EUR 304

Mouse pre-microRNA Expression Construct mir-488
MMIR-488-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

GPR12
RA30050 50 ug
EUR 618

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)
43R-ID012AF 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated
Ab8011-001 1mg
EUR 334

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647
A6-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647
A6-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647
A6-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647
A6-737-C100 0.1 mg
EUR 269

Gpr12/ Rat Gpr12 ELISA Kit
ELI-09764r 96 Tests
EUR 886

GPR12 Conjugated Antibody
C44910 100ul
EUR 397

GPR12 Polyclonal Antibody
ABP54586-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody
ABP54586-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody
ABP54586-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody
ES5585-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody
ES5585-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

Anti-GPR12 antibody
STJ93328 200 µl
EUR 197
Description: Rabbit polyclonal to GPR12.

GPR12 siRNA
20-abx902268
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA
20-abx918427
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA
20-abx918428
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

Anti-GPR12/Gpcr12 Antibody
A10818 100ul
EUR 397
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human.

GPR12 Antibody, HRP conjugated
1-CSB-PA009742LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated
1-CSB-PA009742LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated
1-CSB-PA009742LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)
50337 0.5 ml
EUR 225

GPR12 Blocking Peptide
DF2721-BP 1mg
EUR 195

GPR12 Blocking Peptide
20-abx062679
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

Polyclonal GPR12 Antibody (Extracellular Domain)
APR16480G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)
APR16481G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

GPR12 ELISA KIT|Human
EF005024 96 Tests
EUR 689

Rat GPR12 shRNA Plasmid
20-abx986567
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human GPR12 shRNA Plasmid
20-abx951900
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse GPR12 shRNA Plasmid
20-abx970621
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

GPR12 Recombinant Protein (Human)
RP061035 100 ug Ask for price

GPR12 Recombinant Protein (Rat)
RP203285 100 ug Ask for price

GPR12 Recombinant Protein (Rat)
RP203288 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)
RP139421 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)
RP139424 100 ug Ask for price

Polyclonal GPR12 Antibody - C-terminal region
APR16482G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

Phosphoserine Antibody (ATTO 488)
abx448295-400ul 400 ul
EUR 592
  • Shipped within 5-12 working days.

Phosphothreonine Antibody (ATTO 488)
abx448296-400ul 400 ul
EUR 592
  • Shipped within 5-12 working days.

Phosphotyrosine Antibody (ATTO 488)
abx448417-400ul 400 ul
EUR 592
  • Shipped within 5-12 working days.

Crotonaldehyde Antibody (ATTO 488)
abx448590-100ug 100 ug
EUR 704
  • Shipped within 5-12 working days.

Crotonaldehyde Antibody (ATTO 488)
abx448608-100ug 100 ug
EUR 704
  • Shipped within 5-12 working days.

KDEL Antibody (ATTO 488)
abx448662-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

KDEL Antibody (ATTO 488)
abx448680-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

KDEL Antibody (ATTO 488)
abx448698-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

GRP94 Antibody (ATTO 488)
abx448716-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Ubiquitin Antibody (ATTO 488)
abx448824-100ug 100 ug
EUR 662
  • Shipped within 5-12 working days.

Ubiquitin Antibody (ATTO 488)
abx448842-100ug 100 ug
EUR 662
  • Shipped within 5-12 working days.

GST Antibody (ATTO 488)
abx448860-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Ubiquitin Antibody (ATTO 488)
abx448878-100ug 100 ug
EUR 662
  • Shipped within 5-12 working days.

Metallothionein Antibody (ATTO 488)
abx448896-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Metallothionein Antibody (ATTO 488)
abx448914-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Metallothionein Antibody (ATTO 488)
abx448932-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Tbj51 Antibody (ATTO 488)
abx448968-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

TbHsp70 Antibody (ATTO 488)
abx448986-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

CBP Antibody (ATTO 488)
abx449058-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

CBP Antibody (ATTO 488)
abx449076-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

SMAD3 Antibody (ATTO 488)
abx449112-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

SFRP1 Antibody (ATTO 488)
abx449310-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

PDGFB Antibody (ATTO 488)
abx449400-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

BAD Antibody (ATTO 488)
abx449454-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

BAD Antibody (ATTO 488)
abx449472-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

CDKN2A Antibody (ATTO 488)
abx449490-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

MEK1 Antibody (ATTO 488)
abx449562-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

4EBP1 Antibody (ATTO 488)
abx449598-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

LRP1 Antibody (ATTO 488)
abx449652-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

LRP1 Antibody (ATTO 488)
abx449670-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

SFRP2 Antibody (ATTO 488)
abx449688-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

DKK1 Antibody (ATTO 488)
abx449706-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

FOXO1 Antibody (ATTO 488)
abx449760-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

PDGFB Antibody (ATTO 488)
abx449796-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Palladin Antibody (ATTO 488)
abx449850-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

RICTOR Antibody (ATTO 488)
abx449886-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Kir4.1 Antibody (ATTO 488)
abx445822-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody
20-abx009208
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody
20-abx147559
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody
20-abx324375
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody
20-abx338465
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gpr12 ORF Vector (Rat) (pORF)
ORF067763 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Rat) (pORF)
ORF067764 1.0 ug DNA
EUR 506

GPR12 ORF Vector (Human) (pORF)
ORF020346 1.0 ug DNA
EUR 405

Gpr12 ORF Vector (Mouse) (pORF)
ORF046475 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Mouse) (pORF)
ORF046476 1.0 ug DNA
EUR 506

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a 
RP-488 100 ug
EUR 286

Tide Fluor 2-LL-37
H-8286.0100 0.1mg
EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37
H-8286.0500 0.5mg
EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Anti-Cytokeratins Alexa Fluor488
A4-108-C025 0.025 mg
EUR 175

Anti-Cytokeratins Alexa Fluor488
A4-108-C100 0.1 mg
EUR 310

Anti-PSMA Alexa Fluor488
A4-539-C025 0.025 mg
EUR 227

Anti-PSMA Alexa Fluor488
A4-539-C100 0.1 mg
EUR 414

Anti-FoxP3 Alexa Fluor488
A4-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor488
A4-601-C100 0.1 mg
EUR 362

Anti-Phosphotyrosine Alexa Fluor647
A6-263-C025 0.025 mg
EUR 154

Anti-Phosphotyrosine Alexa Fluor647
A6-263-C100 0.1 mg
EUR 269

Anti-LCK Alexa Fluor647
A6-269-C025 0.025 mg
EUR 206

Anti-LCK Alexa Fluor647
A6-269-C100 0.1 mg
EUR 373

Anti-FoxP3 Alexa Fluor647
A6-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor647
A6-601-C100 0.1 mg
EUR 362

Bassoon (BSN) Antibody (ATTO 488)
abx446587-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Rubicon (Rubicon) Antibody (ATTO 488)
abx446723-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Endoplasmin (GRP94) Antibody (ATTO 488)
abx447029-100ug 100 ug
EUR 537
  • Shipped within 5-12 working days.

PUMA (NT) Antibody (ATTO 488)
abx447454-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

PUMA (CT) Antibody (ATTO 488)
abx447471-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Piccolo (Pclo) Antibody (ATTO 488)
abx447488-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

ubiquitin (Ubiquitin) Antibody (ATTO 488)
abx447947-200ul 200 ul
EUR 634
  • Shipped within 5-12 working days.

STI1P (HOP) Antibody (ATTO 488)
abx448168-100ul 100 ul
EUR 551
  • Shipped within 5-12 working days.

Metallothionein (mymT) Antibody (ATTO 488)
abx448203-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

DYKDDDDK Tag Antibody (ATTO 488)
abx448204-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Acetylated Lysine Antibody (ATTO 488)
abx448297-100ug 100 ug
EUR 592
  • Shipped within 5-12 working days.

Methylated Lysine Antibody (ATTO 488)
abx448298-100ug 100 ug
EUR 592
  • Shipped within 5-12 working days.

c-Fos Antibody (ATTO 488)
abx448734-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

c-Fos Antibody (ATTO 488)
abx448752-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

c-Fos Antibody (ATTO 488)
abx448770-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

c-Fos Antibody (ATTO 488)
abx448788-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

c-Fos Antibody (ATTO 488)
abx448806-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Hsp90 alpha Antibody (ATTO 488)
abx448950-100ug 100 ug
EUR 634
  • Shipped within 5-12 working days.

Axin-1 Antibody (ATTO 488)
abx449004-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Axin-2 Antibody (ATTO 488)
abx449022-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Axin-2 Antibody (ATTO 488)
abx449040-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

c-Jun Antibody (ATTO 488)
abx449094-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Presenilin 1 Antibody (ATTO 488)
abx449130-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

GSK3 beta Antibody (ATTO 488)
abx449184-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Frizzled 7 Antibody (ATTO 488)
abx449238-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

PKC gamma Antibody (ATTO 488)
abx449616-100ug 100 ug
EUR 606
  • Shipped within 5-12 working days.

Endoplasmin (GRP94) Antibody (ATTO 488)
abx440283-200ug 200 ug
EUR 592
  • Shipped within 5-12 working days.

GRP75 (Grp75) Antibody (ATTO 488)
abx440303-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

ubiquitin (Ubiquitin) Antibody (ATTO 488)
abx440324-200ug 200 ug
EUR 634
  • Shipped within 5-12 working days.

ubiquitin (Ubiquitin) Antibody (ATTO 488)
abx440333-200ug 200 ug
EUR 634
  • Shipped within 5-12 working days.

Rhodopsin (RHO) Antibody (ATTO 488)
abx440336-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Rhodopsin (RHO) Antibody (ATTO 488)
abx440337-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

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