myology2019

Detection and quantification of EGFR T790M mutation

Posted on by Lydia

Detection and quantification of EGFR T790M mutation in liquid biopsies by droplet digital PCR

 Background: Liquid biopsy allows the identification of targetable cancer mutations in a minimally invasive manner. In patients with advanced non-small cell lung cancer (NSCLC), droplet digital PCR (ddPCR) is increasingly used to genotype the epidermal growth factor receptor (EGFR) gene in circulating cell-free DNA (cfDNA). However, the sensitivity of this method is still under debate. The aim of this study was to implement and assess the performance of a ddPCR assay for detecting the EGFR T790M mutation in liquid biopsies.

 Methods: A ddPCR assay was optimized to detect the EGFR T790M mutation in plasma samples from 77 patients with NSCLC in progression.

 Results: Our ddPCR assay enabled the detection and quantification of the EGFR T790M mutation at cfDNA allele frequency as low as 0.5%. The mutation was detected in 40 plasma samples, corresponding to a positivity rate of 52%.

The number of mutant molecules per mL of plasma ranged from 1 to 6,000. A re-biopsy was analyzed for 12 patients that had a negative plasma test and the mutation was detected in 2 cases. A second liquid biopsy was performed for 6 patients and the mutation was detected in 3 cases.

Conclusions: This study highlights the value of ddPCR to detect and quantify the EGFR T790M mutation in liquid biopsies in a real-world clinical setting. Our results suggest that repeated ddPCR tests in cfDNA may obviate tissue re-biopsy in patients unable to provide a tumor tissue sample suitable for molecular analysis.

A rapid and sensitive fluorescence biosensor based on plasmonic PCR

  •  Plasmonic PCR utilizing metallic nanoparticles has shown great advantages compared to the commercial thermocycler equipment in terms of cost, size and processing time. However, due to the strong fluorescence quenching, plasmonic nanoparticle-based PCR requires additional post-processing steps such as centrifugation and gel electrophoresis.
  • This process increases the overall diagnostic time, offsetting the benefits of fast thermocycling. Here, we report a rapid and sensitive plasmonic photothermal PCR (PPT-PCR) assay method based on in situ end-point fluorescence detection.
  • By using plasmonic magnetic bi-functional nanoparticles, PPT-PCR involving 30 thermocycles and fluorescence detection following magnetic separation has successfully shown that DNA targets can be detected within 5.5 minutes.
  • The limit of detection (3.3 copies per μL) is comparable with that of the conventional real-time quantitative PCR; however, the assay time is about 5.5 times shorter for the PPT-PCR. The strategy of combining the photothermal effect and magnetic separation into a single particle will open new horizons in the development of fast and sensitive PCR-based biosensors for point-of care testing.
myology2019
myology2019

Comorbidities and Age Are Associated With Persistent COVID-19 PCR Positivity

 Objectives: The impact of demographics and comorbidities on the duration of COVID-19 nasopharyngeal swab PCR positivity remains unclear. The objective of our analysis is to determine the impact of age, intensive care unit (ICU) admission, comorbidities, and ethnicity on the duration of COVID-19 PCR positivity among hospitalized patients in a large group of hospital.

 Method: We studied 530 patients from a large hospital system and time to SARS-CoV-2 virus RNA PCR negativity at any-time during hospitalization or following discharge from the hospital was the primary endpoint. We included patients 18 years or older who tested positive for COVID-19 during an inpatient, outpatient, or emergency room visit between February 1, 2020, and April 14, 2020.

 Results: Overall, 315 (59.4%) of our patient population continued to have a positive SARS-CoV-2 virus RNA PCR 4 weeks after the initial positive test. We found that age>70 years, chronic kidney disease, hypertension, hyperlipidemia, obesity, or coronary artery disease are associated with persistent PCR positivity for more than 4 weeks after initial diagnosis.

 Conclusion: Age, and the presence of co-morbidities should be taken into consideration when interpreting a positive COVID PCR test.

Comparison of PCR and phenotypic methods for the detection of methicillin resistant Staphylococcus aureus

 Background and objectives: Resistance to methicillin in methicillin resistant strains of Staphylococcus aureus (MRSA) is due to the presence of mec-A gene, which encodes a low affinity penicillin binding protein (PBP)-2a or PBP2. Accurate and rapid identification of MRSA in clinical specimens is essential for timely decision on effective treatment. The aim of the study was to compare three different methods for detection of MRSA namely cefoxitin disc diffusion, CHROM agar MRSA and VITEK-2 susceptibility with PCR which is the gold standard reference method and to find the antibiotic susceptibility pattern of these isolates by VITEK-2.

 

Materials and methods: A Total of 100 non-duplicate S. aureus isolates were collected from different clinical samples among both outpatient and inpatients. Detection of MRSA among these isolates was done by cefoxitin disc diffusion, VITEK-2, CHROM agar MRSA and PCR.

 Results: The sensitivity and specificity of cefoxitin disc diffusion and Vitek was found to be 97.2% and 100%, while that of CHROM agar was found to be 100% and 78.6%. The overall prevalence of MRSA in our study by PCR was 72%.

 

Conclusion: Based on the findings in our study, isolates which show cefoxitin zone diameter < 22 mm can be reported as MRSA. However, those isolates which have a zone diameter between 22-24 mm, should ideally be confirmed by PCR.

XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluor™ 594*
11120160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluor™ 594*
11120161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*
45035 100 Slides
EUR 334

XFD594 Anti-human CD133 Antibody *293C3, XFD594 Same Structure to Alexa Fluor™ 594*
11330160 100 tests
EUR 607
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD133 Antibody *293C3, XFD594 Same Structure to Alexa Fluor™ 594*
11330161 500 tests
EUR 2280
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD117 Antibody *104D2, XFD594 Same Structure to Alexa Fluor™ 594*
11170170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD117 Antibody *104D2, XFD594 Same Structure to Alexa Fluor™ 594*
11170171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

anti-GFP VHH antibody (Alexa Fluor® 647)
JOT0001-3 each Ask for price

anti-GFP VHH antibody (Alexa Fluor®488)
JOT0001-4 each Ask for price

anti-GFP VHH antibody (Alexa Fluor®568)
JOT0001-5 each Ask for price

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*
10640170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*
10640171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluor™ 594*
10281170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluor™ 594*
10281171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluor™ 594*
10042170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluor™ 594*
10042171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*
10081170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*
10081171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluor™ 594*
10033170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluor™ 594*
10033171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD82 Antibody *C33, XFD594 Same Structure to Alexa Fluor™ 594*
10820170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD82 Antibody *C33, XFD594 Same Structure to Alexa Fluor™ 594*
10820171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluor™ 594*
10720170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluor™ 594*
10720171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluor™ 594*
10730170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluor™ 594*
10730171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*
10113170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*
10113171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*
10161170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*
10161171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *SN13, XFD594 Same Structure to Alexa Fluor™ 594*
10012160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *SN13, XFD594 Same Structure to Alexa Fluor™ 594*
10012161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *L161, XFD594 Same Structure to Alexa Fluor™ 594*
10013160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *L161, XFD594 Same Structure to Alexa Fluor™ 594*
10013161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*
10090170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*
10090171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluor™ 594*
10193170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluor™ 594*
10193171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluor™ 594*
10194160 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluor™ 594*
10194161 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*
10070170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*
10070171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD32 Antibody *3D3, XFD594 Same Structure to Alexa Fluor™ 594*
10321170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD32 Antibody *3D3, XFD594 Same Structure to Alexa Fluor™ 594*
10321171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD38 Antibody *HB7, XFD594 Same Structure to Alexa Fluor™ 594*
10382160 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD38 Antibody *HB7, XFD594 Same Structure to Alexa Fluor™ 594*
10382161 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD45 Antibody *2D1, XFD594 Same Structure to Alexa Fluor™ 594*
10454170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD45 Antibody *2D1, XFD594 Same Structure to Alexa Fluor™ 594*
10454171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD40 Antibody *G28.5, XFD594 Same Structure to Alexa Fluor™ 594*
10401171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD93 Antibody *VIMD2, XFD594 Same Structure to Alexa Fluor™ 594*
10930170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD93 Antibody *VIMD2, XFD594 Same Structure to Alexa Fluor™ 594*
10930171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD95 Antibody *EOS9.1, XFD594 Same Structure to Alexa Fluor™ 594*
10950170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD95 Antibody *EOS9.1, XFD594 Same Structure to Alexa Fluor™ 594*
10950171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD99 Antibody *HI156, XFD594 Same Structure to Alexa Fluor™ 594*
10990170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD99 Antibody *HI156, XFD594 Same Structure to Alexa Fluor™ 594*
10990171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD99 Antibody *HIT4, XFD594 Same Structure to Alexa Fluor™ 594*
10991170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD99 Antibody *HIT4, XFD594 Same Structure to Alexa Fluor™ 594*
10991171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD56 Antibody *My31, XFD594 Same Structure to Alexa Fluor™ 594*
10562160 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD56 Antibody *My31, XFD594 Same Structure to Alexa Fluor™ 594*
10562161 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD57 Antibody *HI57a, XFD594 Same Structure to Alexa Fluor™ 594*
10570170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD57 Antibody *HI57a, XFD594 Same Structure to Alexa Fluor™ 594*
10570171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD58 Antibody *HI58a, XFD594 Same Structure to Alexa Fluor™ 594*
10580170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD58 Antibody *HI58a, XFD594 Same Structure to Alexa Fluor™ 594*
10580171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD62 Antibody *HI62E, XFD594 Same Structure to Alexa Fluor™ 594*
10620170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD62 Antibody *HI62E, XFD594 Same Structure to Alexa Fluor™ 594*
10620171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD69 Antibody *FN50, XFD594 Same Structure to Alexa Fluor™ 594*
10690170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD69 Antibody *FN50, XFD594 Same Structure to Alexa Fluor™ 594*
10690171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD84 Antibody *CD84.1.21, XFD594 Same Structure to Alexa Fluor™ 594*
10840170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD84 Antibody *CD84.1.21, XFD594 Same Structure to Alexa Fluor™ 594*
10840171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD85 Antibody *17G10.2, XFD594 Same Structure to Alexa Fluor™ 594*
10850170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD85 Antibody *17G10.2, XFD594 Same Structure to Alexa Fluor™ 594*
10850171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD87 Antibody *VIM5, XFD594 Same Structure to Alexa Fluor™ 594*
10870170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD87 Antibody *VIM5, XFD594 Same Structure to Alexa Fluor™ 594*
10870171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD71 Antibody *HI160, XFD594 Same Structure to Alexa Fluor™ 594*
10710170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD71 Antibody *HI160, XFD594 Same Structure to Alexa Fluor™ 594*
10710171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD71 Antibody *HI166, XFD594 Same Structure to Alexa Fluor™ 594*
10711170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD71 Antibody *HI166, XFD594 Same Structure to Alexa Fluor™ 594*
10711171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-mouse CD197 Antibody *4B12, XFD594 Same Structure to Alexa Fluor™ 594*
11970160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-mouse CD197 Antibody *4B12, XFD594 Same Structure to Alexa Fluor™ 594*
11970161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD205 Antibody *HD30, XFD594 Same Structure to Alexa Fluor™ 594*
12050160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD205 Antibody *HD30, XFD594 Same Structure to Alexa Fluor™ 594*
12050161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD209 Antibody *UW60.1, XFD594 Same Structure to Alexa Fluor™ 594*
12090170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD209 Antibody *UW60.1, XFD594 Same Structure to Alexa Fluor™ 594*
12090171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD229 Antibody *HLy9.25, XFD594 Same Structure to Alexa Fluor™ 594*
12290170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD229 Antibody *HLy9.25, XFD594 Same Structure to Alexa Fluor™ 594*
12290171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD231 Antibody *B2D, XFD594 Same Structure to Alexa Fluor™ 594*
12310170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD231 Antibody *B2D, XFD594 Same Structure to Alexa Fluor™ 594*
12310171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD235 Antibody *HIR2, XFD594 Same Structure to Alexa Fluor™ 594*
12351170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD235 Antibody *HIR2, XFD594 Same Structure to Alexa Fluor™ 594*
12351171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD243 Antibody *UIC2, XFD594 Same Structure to Alexa Fluor™ 594*
12430170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

Universal primers for rapid detection of six pospiviroids in Solanaceae plants using one-step RT-PCR and RT-LAMP

A number of viruses and viroids infect solanaceous plants causing severe yield losses. Several seed-borne viroids are currently listed as quarantine pathogens in many countries. Among them, columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd), tomato apical stunt viroid (TASVd), tomato chlorotic dwarf viroid (TCDVd), and tomato planta macho viroid (TPMVd) are of major concerns.

The objective of this study was to design and test universal primers that could be used to detect six viroids in solanaceous plants using one-step RT-PCR and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Results revealed that a pair of degenerate primers could be used in a one-step RT-PCR to amplify six pospiviroids from Solanaceae seeds and plants. Moreover, five primers were designed and used in RT-LAMP to amplify six pospiviroids.

The minimal concentration of viroid RNA required for a successful detection varied, ranging from one femtogram to 10 nanograms, depending on the species of viroid and detection method. In general, RT-LAMP was more sensitive than RT-PCR but both assays were rapid and highly sensitive tools to detect six pospiviroids.

Detection methods currently in use for these viroids require at least two different sets of primers. The assays developed in this research could facilitate to screen a large number of solanaceous plants and seeds intended for import and export.

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