The Glutathione S-transferase Activity Research Exclusive Use Kit is a fluorescent activity assay designed for the quantification and detection of glutathione S-transferase activity in serum, plasma, urine, and cell lysates.
This complete, ready-to-use kit includes 96-well black plates, glutathione S-transferase standard (10 U / ml), glutathione S-transferase substrate, and other components to perform the assay. A 96-well microplate reader capable of reading fluorescent emission at 460 nm, with excitation at 370-410 nm, is required to use this kit.
• Assay type: fluorescent activity kit
• Types of samples: serum, plasma, urine, and cell lysates
• Sensitivity: 2.70 mU / mL
• Standard curve range: 7.8 mU / mL – 500 mU / mL
• Reactivity: independent species
The glutathione S-transferase (GST) family of isoenzymes functions to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione1. Human GSTs are encoded by 5 gene families, expressed in almost all tissues as four cytosolic and one microsomal form.
When dividing the family by isoelectric points, the basic alpha (pI 8-11), neutral mu (pI 5-7), and acidic pi (pH <5) classes are populated by additional subclasses, each isozyme showing differential specificity for an electrophile. . dice. molecules. This assay has been validated for human urine, serum, EDTA, heparin plasma, toadfish liver (Opsanus tau), and oyster hemolymph samples. Most cell lysates should also be compatible. GST activity varies by tissue and species, therefore this kit should measure GST activity from sources other than humans.
The Fluorescent Glutathione S-transferase Activity Kit is designed to quantitatively measure GST activity present in a variety of samples. A GST standard is provided to generate a standard curve for the assay and all samples should be read from the standard curve.
The kit uses a non-fluorescent molecule that is a substrate for the GST enzyme that covalently binds to glutathione (GSH) to produce a highly fluorescent product. Mixing the sample or standard with the supplied detection reagent and GSH and incubating at room temperature for 30 minutes produces a fluorescent product that is read at 460 nm on a fluorescent plate reader with excitation at 390 nm.